In situ in situ and ex situ ex situ bioremediation technologies for groundwater contaminated with chlorinated solvents michelle m. It also suffers from costs associated with solid handling process e. This is the key difference between in situ and ex situ bioremediation. Emily majcher, duane graves, geosyntec consultants. Ex situ bioremediation occurs at a location separate from the contaminated site and involves the translocation of the contaminated material. Pdf ex situ bioremediation of soil contaminated with. These tests may be performed during the feasibility study or remedial design, are used to evaluate whether the proposed bioremediation remedy will be successful,and obtain important design criteria. Groundwater remediation 2 1 introduction groundwater remediation is the cleanup of contaminated groundwater to levels that are in compliance with regulatory limits. This article discusses the design, operation, performance, and modifications to the remediation system over time. Bioremediation relies on microbes that live naturally in soil and groundwater. In situ bioremediation is the application of biological treatment to the cleanup of hazardous chemicals present in the subsurface. Dec 10, 2017 in situ and ex situ bioremediation technologies p1 duration. Introduction to in situ bioremediation of groundwater.
Liens p, grotenhuis t, tabak h eds soil and sediment remediation. Ex situ techniques can be faster, easier to control, and used to treat a wider range of contaminants and soil types than in situ techniques. In this the contaminated soil is excavated and removed from the site as completely as possible. Trick to learn in situ on site and ex situ off site conservation duration. Ex situ bioremediation method for the treatment of groundwater. Ex situ biological treatment one of the main advantages of ex situ bioremediation is that it requires less time than the in situ treatment. Ex situ bioremediation approaches include use of bioreactors, landfarming, and biopiles. Bioremediation processes are cost effective, safe and naturebased methods over the chemical and physical methods. This section discusses in situ and ex situ bioremediation. The characteristics of bioremediation projects at superfund and other sites are described in section 3. Rather, both in situ and ex situ methods are effective, depending on the facilityspecific characteristics and the nature of the application. In situ and ex situ characterization of the microstructure. In situ bioremediation involves treating the contaminated material at the site, while ex situ involves the removal of the contaminated material to be treated elsewhere.
The potential effects of sawdust, and mixture of cow and sheep dung to biostimulant autochthonous microflora and augmentation for hydrocarbon bioremediation were investigated in test biopile, made of soil polluted with petroleum waste 100kg. Sections 5, 7, 8, 9, and 17 of this policy do not apply to pilot scale facilities. Bioremediation as a sustainable alternative to chemicalphysical processes is an attractive solution for soil decontamination and renewal of polluted sites. Sep 17, 2018 the main difference between in situ and ex situ is that the in situ refers to the original location whereas the ex situ refers to the offsite. Six pumping wells, two recharge wells and fifteen observation wells were installed for this study as a part of the ex situ biodegradation treatment plant. The detailed results of ex situ controlled aerobic bioremediation of ten sites with differing petroleum contamination and four in situ cases are reported and compared. In situ conservation means the conservation of ecosystems and natural habitats and the maintenance and recovery of viable populations of species in their natural surround. In situ bioremediation of groundwater has become one of the most widely used technologies for contaminated site treatment because of its relatively low cost, adaptability to site specific conditions, and efficacy when properly implemented. Some examples of bioremediation technologies are bioaugmentation, biostimulation, bioreactors, landbased treatments, and fungal remediation. Case study of ex situ remediation and conversion to a. A general essay on bioremediation of contaminated soil.
Difference between in situ and ex situ conservation. Generally, bioremediation technologies can be classified as in situ or ex situ. Bioremediation techniquesclassification based on site of. Phytoremediation acknowledgements glossary bibliography biographical sketch summary a brief outline of the development of bioremediation technologies is presented. The soil can, however, be used for landscaping purposes. Bioremediation is a process that uses microorganisms or their enzymes to remove pollutants from the environment. Fundamental principles of bioremediation an aid to the. Combined exsitu and insitu remediation of soil and. Combined exsitu and insitu remediation of soil and groundwater impacts to meet site closure requirements presented by. Ex situ bioremediation, in which contaminated soil is excavated and treated elsewhere, is an alternative. Another advantage is the certainty of the control treatment due to the ability to uniformly screen, homogenize and mix the soil. Furthermore, in situ methods are less expensive and less manageable while the ex situ methods are expensive and manageable.
Ex situ bioremediation ex situ bioremediation involves removal of waste materials and their collection at a place to facilitate microbial degradation. Ex situ conservation literally means, offsite conservation. Bioremediation that can occur on its ownfactors effecting rate of intrinsicbioremediation. In situ bioremediation involves treating the polluted material at the site while ex situ involves the removal of the polluted material to be treated elsewhere aggarwal et al. Ex situ bioremediation involves extraction of contaminated media to other location for treatment. Ex situ bioremediation list of high impact articles ppts. The use of organisms to reduce and transform contaminants into nontoxic substances is an environmentally friendly process which does not ne. When consider bioremediation, it becomes an ex situ process when the pollutants are collected from their natural location for the treatment. A major advantage of ex situ bioremediation processes is that most of the decontaminated soil can be reused. In situ occurs within the site of contamination 1 in situ bioremediation can further be categorized by the metabolism occurring, aerobic and anaerobic, and by the level of human involvement. Ex situ conservation provides less mobility to the organisms due to relatively smaller habitat or area than the in situ. Applicable for both warm and cold temperatureclimate site applications. It is the process of protecting an endangered species, variety or breed, of plant or animal outside its natural habitat.
In situ and ex situ application processes to liberate contaminants making them more bioavailable for microbial bacteria degradation. Pdf ex situ bioremediation of soil contaminated with crude. Fundamental principles of bioremediation an aid to. Upon cooling, organic contaminants are pyrolysed and. Ex situ bioremediation technology includes most of disadvantages and limitations. In aerobic bioventing, air is typically injected into the subsurface to facilitate aerobic metabolism of hydrocarbons. Gk, general studies, optional notes for upsc, ias, banking, civil services. Bioremediation technologies can be generally classified as in situ or ex situ. In situ and ex situ bioremediation technologies p2 duration. Later, the air stripper and carbon units were eliminated, and an infiltration gallery was installed to receive treated, oxygenated effluent in order to promote flushing of the smear zone and in situ bioremediation in the source area. Ex situ bioremediation techniques are unlikely to be used in some sites such as under buildings, inner city and working sites philp and atlas 2005. These tests may be performed during the feasibility study or remedial design, are used to evaluate whether the proposed bioremediation remedy will be successful, and obtain important design criteria. The first commercial in situ bioremediation system was installed in 1972 to. Ex situ bioremediation of soil contaminated with crude oil by.
Bioremediation approaches are generally classified as in situ or ex situ. It is concluded, that bioremediation of petroleum contaminated soil is an effective, safe and economically feasible remediation technique for the remediation of such. Coupling bioflocculation of dehalococcoides to highdechlorination rates for ex situ and in situ bioremediation abstract bioremediation of trichloroethene tce using dehalococcoides mccartyicontaining microbial cultures is a recognized and successful remediation technology. This technique is usually faster, easier to control and used to treat a wider range soil types and contaminants than in situ techniques. Engineering issue in situ and ex situ biodegradation. In ex situ bioremediation, contaminants are treated in some other place from the original site.
The design process commonly includes bench and pilotscale treatability studies. Pdf on sep 14, 2014, s raja and others published ex situ and in situ bioremediation strategies with special reference to marine and coastal environments find, read and cite all the research. It consists of placing the excavated materials into an above ground enclosure. The optimization and control of microbial transformations of organic contaminants require the integration of many scientific and engineering disciplines. Mahdi 1biology department, college of science, university of basrah, basrah, iraq. In in situ bioremediation, contaminants are treated at the same site using biological systems. These microbes pose no threat to people at the site or in the community. Bioremediation experts representing academic research, field practice, regulation, and industry provide accessible information and case examples.
Bioremediation techniques are designed to break down contaminants via the stimulation of microorganisms which use the contamination as an energy source for development and growth. Ex situ bioremediation processes involve an excavation of contaminated soil or pumping of groundwater before and sometimes, after the actual bioremediation treatment. An overview of conventional and innovative technologies. The goal in evaluating in situ bioremediation is to assess whether the weight of evidence from tests such as those described above documents a convincing case for successful bioremediation. However, they require excavation and treatment of the contaminated soil before and, sometimes, after the actual bioremediation step. Introduction to in situ bioremediation of groundwater es3. Increasingly, in situ bioremediation is being heralded as a promising new alternative ground water cleanup technology. Some examples of bioremediationrelated technologies are phytoremediation, bioventing, bioleaching, landfarming, bioreactor, composting, bioaugmentation, rhizofiltration, and. All of these types of bioremediation are receiving increasing attention as viable remediation alternatives for several reasons. There is more certainty about the uniformity of treatment because of the ability to homogenize, screen, and continuously mix the soil. Ex situ bioremediation treatment landfarming springerlink. Sep 16, 2016 ex situ bioremediation techniques are unlikely to be used in some sites such as under buildings, inner city and working sites philp and atlas 2005.
Mar 03, 2017 key difference in situ vs ex situ bioremediation bioremediation is a term used in biotechnology to refer the process of cleaning the polluted areas using biological organisms such as microorganisms and plants. Technologies grace as part of a grant to complete development of has been applied to both ex situ and in situ treatment of soil. In situ bioremediation an overview sciencedirect topics. Various bioremediation techniques can be applied in situ or ex situ and under aerobic or anaerobic conditions. Pdf on sep 14, 2014, s raja and others published exsitu and insitu bioremediation strategies with special reference to marine and coastal environments find, read and cite all the research. Examples of ex situ processes include land treatment and composting.
Coupling bioflocculation of dehalococcoides to high. And, ex situ bioremediation means you would scoop up the contaminated soil or pump up the contaminated water and take it somewhere else for bioremediation. Dec 10, 2017 in situ and ex situ bioremediation technologies p2 duration. In the use of a bioreactor, contaminated soil is mixed with water and nutrients and the mixture is agitated by a mechanical bioreactor to stimulate. Similarities between in situ and ex situ in situ and ex situ are two phenomena of many biological processes classified based on the location where the process is carried out. Insitu and exsitu bioremediation technologies p1 duration. Investigators cannot fully understand the details of whether and how bioremediation is occurring at a site. Ex situ bioremediation of soil ex situ techniques can be faster, easier to control, and used to treat a wider range of contaminants and soil types than in situ techniques. Ex situ bioremediation environmental biotechnology. National parks, sacred forests, lakes, marine parks, biosphere reserves, gene sanctuaries. In situ and ex situ bioremediation technologies p2 youtube.
Pdf exsitu and insitu bioremediation strategies with. Us epa engineering issue in situ and ex situ biodegradation. Often a synergistic remedial effect can be attained by using a combination of methods, such as ex situ methods to address grossly contaminated source soils or groundwater. Section 2 of the report provides an overview of bioremediation technologies, including in situ and ex situ technologies, and provides examples of field use for three types of bioremediation technologies. Exsitu biological treatment one of the main advantages of exsitu bioremediation is that it requires less time than the insitu treatment. In situ and ex situ are two methods used to describe different biological processes such as. In situ bioremediation the use of microorganisms for onsite removal of contaminantsis potentially cheaper, faster, and safer than conventional cleanup methods.
Historical information on the development of bioremediation can be found in martin and gershuny, 1992. The main difference between in situ and ex situ is that the in situ refers to the original location whereas the ex situ refers to the offsite. Ex situ bioremediation can use bioreactors and added nutrients. The most feasible bioremediation technologies of unsaturated soils contaminated by petroleum are 1 the onsite controlled remediation of the excavated contaminated plume in the contained system. In situ bioremediation environmental biotechnology. Pdf on sep 14, 2014, s raja and others published exsitu and insitu bioremediation strategies with special reference to marine and coastal. Kalogerakis n 2005 ex situ bioremediation of contaminated soils. Biotransformation in the surface environment is a very complex process. The grant was this report addresses the results of the ex situ demonstration at funded by the ontario ministry of environment and energys the novartis site. Difference between in situ and ex situ bioremediation. A systematic approach to in situ bioremediation in groundwater, including decision trees on in situ bioremediation for nitrates, carbon tetrachloride, and perchlorate isb8 aug02 provides guidance for the systematic characterization, evaluation, and appropriate design and testing of isb for any biotreatable contaminant.
Pdf exsitu and insitu bioremediation strategies with special. In situ bioremediation or possibly isco to treat residual hydrocarbon in source area during or following free phase recovery. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the removal of the contaminated material to be treated elsewhere. Some examples of bioremediation related technologies are phytoremediation, bioventing, bioleaching, landfarming, bioreactor, composting, bioaugmentation, rhizofiltration, and. Bioremediation techniques are destruction techniques to stimulate the growth of microorganisms, using the contaminants as a food and energy source. On the other hand, the excavation features of ex situ bioremediation tend to disrupt soil structure. Due to the ex situ techniques used to decontaminate the soil, much of the soil cannot be used as filling or agricultural material. Insitu and exsitu bioremediation technologies p1 youtube. Dec 29, 2011 types of in situ bioremediation1 intrinsic in situ bioremediation. Ex situ processes involve the removal of the contaminated media to a treatment area. Kalogerakis n 2005 exsitu bioremediation of contaminated soils. Ex situ bioremediation describes a process where contaminated soil or water is removed from the environment by biological organisms.
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